Articles | Open Access | DOI: https://doi.org/10.37547/supsci-ojbc-05-02-06%20

ANALYSIS OF INTERSPECIFIC COTTON HYBRIDS USING SSR MARKERS AND IDENTIFICATION OF CHROMOSOME-SUBSTITUTED HYBRIDS

Rukhsora Khalimova ,

Abstract

In Uzbekistan, innovative developments in agricultural production, the introduction of new genetic methods are actively working to create disease-resistant, high-yielding plant varieties. In particular, the Action Strategy for the further development of the Republic of Uzbekistan sets the tasks of "introduction of intensive methods in agricultural production and the creation of new selective varieties adapted to local climatic and environmental conditions." Scientists are stepping up research on these tasks. The reason why G.hirsutum L. type of cotton is in the center of attention of scientists is that the varieties belonging to this species differ from other species by their diversity, but at the same time the varieties belonging to this species have many features in agriculture. may not have sufficient effect in use, including varieties of this type are not superior to varieties of G.barbadense L. type in terms of fiber quality. And its resistance to various environmental conditions and cotton pests poses a new challenge to genetic scientists. G.barbadense L. The genes resc ponsible for fiber length, toughness, and softness are significantly superior to the varieties of G.hirsutum L., which are widely grown in many respects for the fine-grained characteristics of G. barbadense L. Attempts to transfer (introgress) from the genus G. hirsutum L. to the genome of the species by the method of interspecific hybridization are hampered by infertility, disorders of the separation process and limited recombination due to partial mismatch between these two cultivated allotetraploid cotton species.Dess was to identify univalent chromosomes of SSR hybrid plants using SSR markers and cross-linked aneuploid lines of               G. hirsutum L. genus with G. barbadense L. genus Pima 3-79 and which chromosome in hybrids determine the exchange.

Aneuploid lines belonging to the G.hirsutum L. genus of cotton were obtained by cross-mixing F1 hybrid plants with the Pima 3-79 line belonging to the G.barbadense L. species, and identification using SSR markers is the subject of research. DNA separation by STAB method, Gel electrophoresis, PCR, Mathematical statistics, Biomorphological.

Keywords

Cotton (Gossypium hirsutum L.), G.barbadense, interspecific hybridization, fiber quality, cotton breeding, selection, genetic methods, innovative technologies, disease resistance, productivity, earliness, new varieties, intensive methods, scientific research.

References

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Khalimova, R. . (2025). ANALYSIS OF INTERSPECIFIC COTTON HYBRIDS USING SSR MARKERS AND IDENTIFICATION OF CHROMOSOME-SUBSTITUTED HYBRIDS. Oriental Journal of Biology and Chemistry, 5(02), 42–53. https://doi.org/10.37547/supsci-ojbc-05-02-06